von Dassow, George; Verbrugghe, Koen J.C.; Miller, Ann L.; Sider, Jenny R.; Bement, William M. (2021). CIL:13403, Strongylocentrotus purpuratus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TQ6063

16-cell purple urchin embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). 10 µM nocodazole was added at time 00:00. The top left cell entered anaphase around time 0; none of the other cells in view left metaphase nor furrowed. This one cell cleaved despite nearly complete absence of astral microtubules, and the furrow crossed the spindle midzone Purple urchin embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C, which is tolerated well. Corresponds to Fig 3B and third segment of Video 3 in J Cell Biol. 2009 Dec 14;187(6):831-45.

• 2021

• Bement, William M.
• Miller, Ann L.
• Sider, Jenny R.
• Verbrugghe, Koen J.C.
• Von Dassow, George

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 6184

• Strongylocentrotus purpuratus

• Astral microtubule
• Cleavage furrow
• Ensconsin
• Microtubule

• Cytokinesis after mitosis
• Embryo
• Mitotic anaphase
• Nocodazole treatment

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 13403

Doi: https://doi.org/10.6075/J0TQ6063

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13403

Reference

• J Cell Biol. 2009 Dec 14;187(6):831-45. https://www.ncbi.nlm.nih.gov/pubmed/?term=20008563

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2022-08-12