Allen, Richard (2021). CIL:17442, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TM78V8

This EM features the striated bands that link the membranes under the surface depressions of the cortex. The bands insert into the epiplasm, and in this case, they separate to pass around the parasomal sacs which lie to the right of a row of basal body/cilium complexes. The bands have relatively electron transparent zones next to the epiplasm that lie next to more opaque zones which are in turn separated by a central medium electron transparent zone. This differential banding suggests an overlap of filaments in the two more opaque zones, but there is no evidence of a muscle-like actomyosin system. These bands probably provide tension and keep the cortical ridges from flattening. If contractile they could account for the cell's ability to alter its shape when swimming through constricted regions. For details on the striated bands in Paramecium see Allen, J. Cell Biol. 49:1-20, 1971 and Allen, Aihara and Fok, J. Euk . Microbiol. 45:202-209, 1998. This image is adapted with permission from Fig. 14 in J. Cell Biol. 49:1-20, 1971. TEM taken on 12/18/68 by R. Allen with Philips 300 operating at 60kV. Neg. 16,000X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12071) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 4846

• Paramecium multimicronucleatum

• Axoneme
• Cell by organism
• Cell cortex
• Ciliated Protist
• Clathrin coat
• Cortical cytoskeleton
• Cortical striated fiber
• Eukaryotic cell
• Eukaryotic Protist

• Clathrin coat assembly
• Cortical cytoskeleton organization
• Plasma membrane organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 17442

Doi: https://doi.org/10.6075/J0TM78V8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL17442

Reference

• J. Cell Biol. 49:1-20, 1971 https://www.ncbi.nlm.nih.gov/pubmed/?term=4102004

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2025-01-30