Ris, Hans (2021). CIL:41615, Cricetulus griseus, epithelial cell, ovary. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01C1W7T

High Voltage Transmission EM of whole mitotic metaphase chromosomes from Chinese hamster ovary cells. This image was recorded at a specimen tilt of 54 degrees and is grouped with one taken at 46 degrees, creating a stereo pair giving an oblique 3D view of the chromosomes.

• 1977-07-10

• 2021

• Ris, Hans

CHO cells arrested at metaphase with colcemid were treated with 67% D2O, in 0.07M phosphate buffer and squashed in 50% acetic acid. Preparations were transferred to EM grids and examined with the Wisconsin HVEM operated at 1MeV.
See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Preparation: chemically fixed tissue
Relation to intact cell: squash preparation
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 12024

• Cricetulus griseus

• Epithelial cell
• Nuclear chromosome
• Ovary

• CHO
• Chromosome organization
• Mitosis
• Mitotic metaphase

• data
• image

• No linguistic content; Not applicable

Samplenumber: 41615

Doi: https://doi.org/10.6075/J01C1W7T

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL41615

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2023-08-17