Vitha, Stanislav; McAndrew, Rosemary S.; Osteryoung, Katherine W. (2021). CIL:38858, Arabidopsis thaliana, leaf. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0H70DGR

Leaves from the arc5 plastid division mutant of Arabidopsis thaliana were fixed, embedded, sectioned, reacted with antibodies to the FtsZ2-1 protein, a prokaryotic cell division protein that is a structural homolog of tubulin. The protein is localized at the site of division which occurs by constricting at the chloroplast mid-point. The upper image shows the fluorescence signal, the lower panel, the corresponding DIC image.

• 2021

• McAndrew, Rosemary S.
• Osteryoung, Katherine W.
• Vitha, Stanislav

Leaves were fixed in FAA (formalin acetic acid), embedded in low melting point polyester wax, and 7 micrometer sections cut and attached to poly-L-lysine slides. Sections were de-waxed, treated with an antigen retrieval procedure, and processed for immunofluorescence using incubation with an affinity purified polyclonal antibody, followed by FITC-conjugated secondary antibody. Slides were viewed with an Olympus BH2 microscope equipped with DIC optics. Images were recorded on film, or a video camera (Optronics DEI 750 using a 100x 1.25 NA objective lens. See S. Vitha et al. 2001 J Cell Biol 153:111-119.

Preparation: formaldehyde fixed tissue; tissue in wax embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: fluorescence microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of epitope; boundaries between regions with different refractive index
Visualization methods: Fluorescein (FITC)
Data qualification: Processed

• Cell Image Library Group ID: 11385

• Arabidopsis thaliana

• Chloroplast
• Leaf
• Plastid part

• Chloroplast organization
• Plastid organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 38858

Doi: https://doi.org/10.6075/J0H70DGR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL38858

Reference

• S Vitha et al. 2001 FtsZ ring formation at the chloroplast division site in plants. J Cell Biol 153:111-119 https://www.ncbi.nlm.nih.gov/pubmed/?term=11285278

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2022-08-12