Michaels, John E.; Brown, Patricia A. (2021). CIL:10057, Rana catesbeiana, barrier epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0S46R44

This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system. The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and orientation. The coils appear to stack in loose hexagonal arrays (CIL:9301). The plasma membrane of these cells has many caveolae, as illustrated in this micrograph, which also shows several large intermediate filament bundles and desmosomes - insertion sites at the plasma membrane for the intermediate filaments. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).

• 2021

• Brown, Patricia A.
• Michaels, John E.

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: uranyl salt; lead salt; osmium tetroxide
Processing history: unprocessed raw data

• Cell Image Library Group ID: 3044

• Rana catesbeiana

• Barrier epithelial cell
• Intermediate filament cytoskeleton
• Plasma membrane

• Intermediate filament cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10057

Doi: https://doi.org/10.6075/J0S46R44

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10057

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2022-08-12