Sharp, David; Zhang, Dong; Rogers, Gregory; Buster, Daniel (2021). CIL:191, Drosophila melanogaster. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0D799PP

The image shows mitotic metaphase (upper) and anaphase (lower) in Drosophila tissue culture cells immunostained for the microtubule severing protein katanin (green), microtubules (red) and kinetochores/chromosomes (blue). Katanin targets to chromosomes during both metaphase and anaphase and is responsible for inducing the depolymerization of attached microtubule plus-ends.

• 2021

• Buster, Daniel
• Rogers, Gregory
• Sharp, David
• Zhang, Dong

See D. Zhang et al. 2007 Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomes. J Cell Biol 177, 231-242.

Preparation: methanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Processing history: montage
Data qualification: Processed

• Drosophila melanogaster

• Nuclear chromosome
• Spindle

• Mitosis
• S2

• data
• image

• No linguistic content; Not applicable

Samplenumber: 191

Doi: https://doi.org/10.6075/J0D799PP

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL191

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12