Maddox, Paul S.; Hyndman, Francie; Monen, Joost; Oegema, Karen; Desai, Arshad (2021). CIL:28780, Caenorhabditis elegans, embryo. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FB51M9

Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-CeCENP-A in strain OD101. During prophase, discrete foci of GFP signal within the nucleus coalesce to form holocentric kinetochore plates. After nuclear envelope breakdown, chromosomes congress to the metaphase plate and are seen as paired lines. At metaphase, sister kinetochores form two lines facing opposite poles. The video is ∼12 min long, and the frame is ∼30 µm top to bottom. DIC is shown on the left, and GFP is shown on the right. Images were acquired at 10-s intervals.

• 2021

• Desai, Arshad
• Hyndman, Francie
• Maddox, Paul S.
• Monen, Joost
• Oegema, Karen

Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP–γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD camera (Orca ER; Hamamatsu) binning 2 × 2. Strain OD31 expressing GFP–KNL-2 was imaged in the same manner without the 1.5× auxiliary magnification.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization methods: EGFP; visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11164

• Caenorhabditis elegans

• CeCENP-A
• Embryo
• Kinetochore
• Nuclear chromosome

• Metaphase
• Mitosis
• Prophase

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 28780

Doi: https://doi.org/10.6075/J0FB51M9

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL28780

Reference

• J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5. https://www.ncbi.nlm.nih.gov/pubmed/?term=17339379

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2022-08-12