Davis, Margaret I. (2021). CIL:14969, Mus musculus, CNS interneuron, pyramidal cell, basket cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J07W6B91

Distribution of interneurons expressing EGFP from the 5HT3 receptor promoter (Tg(Htr3a-EGFP)DH30Gsat, www.gensat.org) colabelled for the CB1 cannabinoid receptor. This image is from the pyramidal cell layer in hippocampal CA1. Multiple EGFP+ bipolar interneurons are present within the pyramidal cell layer and a mesh of EGFP+ fibers and CB1+ axons is present around the pyramidal cell bodies, consistent with basket cell synapses. EGFP+ cells are also present in the stratum oriens and stratum radiatum. CB1 was detected with the L15 antibody (K. Mackie).

Section (40 um) was imaged with a 32X, 0.4 NA Achroplan objective on a Zeiss Axiovert microscope. AF568 was excited at 562/40 nm and emission collected at 624/40 nm with Semrock filters and a single band dichroic (530-585 nm reflected, 601-800 nm transmitted). AF488 was excited at 482/35 nm and emission collected at 536/40 nm with Semrock filters and a single band dichroic (446-499 nm reflected, 513-735 nm transmitted). DAPI was excited at 350/50 nm and emission collected at 460/50 nm with a CLP400 dichroic (Chroma). Single images were captured for each channel using a Zeiss Axiocam MR CCD and pseudo-colored using Axiovision. See Davis and Puhl 2011 (Pubmed ID 21305052) for a detailed staining protocol. This image is part of a series characterizing EGFP expression from the 5HT3 promoter throughout the brain.

• 2021

• Davis, Margaret I.

Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label; differences in adsorption or binding of stain; distribution of a specific protein
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); Alexa Fluor 568; EGFP
Data qualification: Raw

• Mus musculus

• Axon
• Basket cell
• CNS interneuron
• Dendrite
• Neuronal cell body
• Nucleus
• Pyramidal cell

• Cannabinoid receptor activity
• Cell communication by chemical coupling
• Serotonin receptor activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 14969

Doi: https://doi.org/10.6075/J07W6B91

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL14969

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2022-08-12