CIL:39728
CIL source metadata (JSON)
File Size |
|
File Format |
|
- Collection
- Cite This Work
-
Cell Image Library (2021). CIL:39728. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JW8CNG
- Description
-
TEM images of reconstituted chromatin prepared from 12-mer tandem repeats of a strong nuclear positioning sequence and purified histones. Samples were fixed with glutaraldehyde, applied to carbon films, and shadowed with Pt. (A, B) samples without linker histone H5 fixed in 5 mM NaCl; (C) Sample with H5, fixed in 150 mM NaCl; (D) sample with H5, fixed in 1 mM MgCl2.
See Fig 1 in: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322 - Date Issued
- 2021
- Methods
-
Reconstitutes were fixed in the appropriate salt environment, applied to thin carbon films, air dried, and shadowed with Pt using electron bean evaporation (Balzers BA 080 vacuum evaporator. Grids were examined in a FEI Tecnai 12 TEM at 100KV with a LaB6 filament and tilted darkfield optics, and recorded using a TVIPS 2k CCD camera.
See also: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322. - Technical Details
-
Preparation: glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: CCD
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed - Series
- Anatomy
- Topics
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Identifier
-
Samplenumber: 39728
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39728
- S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322 https://www.ncbi.nlm.nih.gov/pubmed/?term=19651606
- Sergei A. Grigoryev, Gaurav Arya, Sarah Correll, Christopher L. Woodcock, and Tamar Schlick (2009). Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. PNAS. https://doi.org/10.1073/pnas.0903280106
Source data
Reference
- License
-
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
-
Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
-
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12