Ivanchenko, Maryna V.; Cicconet, Marcelo; Jandal, Hoor Al; Wu, Xudong; Corey, David P.; Indzhykulian, Artur A. (2022). CIL: 50728, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0CR5SS5

FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.

• 2022

• Cicconet, Marcelo
• Corey, David P.
• Indzhykulian, Artur A.
• Ivanchenko , Maryna V.
• Jandal, Hoor Al
• Wu, Xudong

Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
Dataset8_MATLAB script is a compressed (zipped) folder containing the MATLAB script files required to perform the referenced study.

Imaging mode: DICM
Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective

• Cell Image Library Group ID: 20008

• Mus musculus

• Cuticular plate
• Hair cell stereocilia
• Inner ear outer hair cell
• Mitochondria
• Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)

• Native auditory hair cells at postnatal day 5

• data
• image

• No linguistic content; Not applicable

Samplenumber: 50728

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL50728

Reference

• Nature Communications article: https://www.nature.com/articles/s41467-019-11712-w

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2022-11-18