LaFountain, James R.; Oldenbourg, Rudolf (2021). CIL:12375, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05T3JP0

Movie showing the dynamics of kinetchore microtubules during meiosis II in primary spermatocytes of the crane-fly Nephrotoma suturalis that were experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 15 sec using a QImaging Retigo EXi CCD camera. Raw images were processed
using 5-frame algorithm (Shribak and Oldenbourg, 2003). The time series used for the movie is included in this grouped set.

• 2021

• LaFountain, James R.
• Oldenbourg, Rudolf

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD); Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm

• Cell Image Library Group ID: 3779

• Nephrotoma
• Nephrotoma suturalis

• Nuclear chromosome
• Primary spermatocyte
• Spindle

• Male meiosis chromosome segregation

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 12375

Doi: https://doi.org/10.6075/J05T3JP0

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12375

Reference

• LaFountain JR, et al. (2004) Mol Cell Biol 15, 5724-5732
• Oldenbourg R, Mei G (1995) J Microsc 180, 140-147
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=12790452
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=8537959
• Shriback M, Oldenbourg R (2003) Appl Opt 42, 3009-3017 https://www.ncbi.nlm.nih.gov/pubmed/?term=15469981

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12