Murray, John; Hu, Ke (2021). CIL:10494, Toxoplasma gondii RH. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00G3JBH

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgIMC1 (innermembrane complex scaffold protein). Images overlaid with DIC. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. Data stack corresponds to CIL# 10495 and deconvolved data stack CIL 10496.
Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

• 2021

• Hu, Ke
• Murray, John

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: fluorescence microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization methods: EGFP; mCherryFP
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 5582

• Toxoplasma gondii RH

• Centriole
• Inner membrane complex
• Polar ring of apical complex

• Cell division
• Regulation of cell shape
• Toxoplasma gondii RH stable cell line
• Toxoplasmosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10494

Doi: https://doi.org/10.6075/J00G3JBH

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10494

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2022-08-12