Korobova, Farida; Svitkina, Tatyana (2021). CIL:40663, Rattus, hippocampal neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0N29VTJ

Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. This image shows branched actin filaments (cyan) at the neck–head junction. Dashed arrows indicate potential filament breakage. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel is available as CIL 40660. The uncolorized image is available as CIL 40659. Box 1, 2, and 3 (this image) are CIL 40661, 40662, and 40663, respectively.

• 2021

• Korobova, Farida
• Svitkina, Tatyana

Please refer to referenced article for details on cell extraction and processing for electron microscopy.

Preparation: detergent permeabilized; glutaraldehyde fixed tissue; critical_point dried specimen
Relation to intact cell: dispersed cells in vitro
Item type: grey scale graphic
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: colorized

• Cell Image Library Group ID: 11349

• Rattus

• Actin cytoskeleton
• Dendritic shaft
• Dendritic spine
• Hippocampal neuron

• Dendrite morphogenesis
• Dendritic spine organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40663

Doi: https://doi.org/10.6075/J0N29VTJ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40663

Reference

• Korobova F, Svitkina T. Molecular architecture of synaptic actin cytoskeleton in hippocampal neurons reveals a mechanism of dendritic spine morphogenesis. Mol Biol Cell. 2010 Jan 1;21(1):165-76. https://doi.org/10.1091/mbc.e09-07-0596
• Mol Biol Cell. 2010 Jan 1;21(1):165-76. Epub 2009 Nov 4. https://www.ncbi.nlm.nih.gov/pubmed/?term=19889835

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2022-08-12