CIL:36702, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:36702, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0DR2TCC
- Description
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Structures at the left edge of the cytopharynx arise in contact with the filamentous reticulum that lies next to the alveoli to the side of the quadrulus. The cytopharyngeal ribbons coming out of the filamentous reticulum have a spacing that seems to be determined by the lattice-like arrangement of this reticulum. A microtubular ribbon arises against each electron-opaque row of nodes of the reticulum. A cytostomal cord borders the cytosolic ends of these ribbons along the entire length of the left side of the cytopharynx. The ribbons curve sharply over the cord and pass out into the endoplasm. Three types of vesicles are transported along these ribbons, discoidal vesicles, large acidosomes and small 100nm carrier vesicles. They all move along the ribbons from the endoplasm toward the cytopharynx and the cytostomal cord toward the origins of the microtubules. Discoidal vesicles reach the cytopharyngeal membrane under the cord where they fuse. Acidosomes are too large to pass under the cord and the 100nm carrier vesicles appear to have an affinity for the acidosomes and fuse with these large vesicles before reaching the cytopharyngeal membrane. TEM taken on 3/28/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 8,500X. Bar = 0.5µm.
- Date Issued
- 2021
- Researcher
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 36702
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36702
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12