Novikoff, Phyllis; Cammer, Michael (2021). CIL:35563, Rattus rattus, duct epithelial cell, hepatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0Q52NKX

Liver of a rat exposed to chemical carcinogens. Serial sections of the liver imaged by laser scanning confocal microscopy. F-actin (red) is in bile canaliculi at junctions of the hepatocytes, which are the large round cells arranged in a circular mass in the bottom center, in ductule cells, which are above and to the left of the hepatocytes, and in blood vessel endothelial cells, which are in the upper right and far left. Fibronectin (green) is in the tissue outside the hepatocytes. Bile ductule and/or oval cells are marked blue.

• 1998-02-20

• 2021

• Cammer, Michael
• Novikoff, Phyllis

The liver of a rat fed a carcinogenic chemicals diet was cryo- and paraformaldehyde-fixed and frozen sections were prepared. The section was labeled with Cy 3 for OV-6, a marker for oval cells/bile ductile cells, Cy5 for fibronectin, and FITC for f-actin. Colors in this image were assigned to each probe by the Cell Imaging Library software. The confocal microscope was a BioRad MRC 600 with a Kr/Ar laser with lines at 488, 568, and 647 nm mounted on a Nikon Diaphot with 60X N.A. 1.4 phase 3 optics. Because the scan head only had two photomultiplier tubes, the filters had to be swapped out manually to collect a series of in register images of more than two fluorescent probes. The microscope was in the Analytical Imaging Facility of the Albert Einstein College of Medicine.

Preparation: cryofixed tissue; chemically fixed tissue; phalloidin; primary antibody plus labeled secondary antibody
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: single point scanning
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Cy5; Cy3; Fluorescein (FITC)
Processing history: channels merged
Data qualification: Processed;spatialmeasurements

• Rattus rattus

• Actin filament
• Duct epithelial cell
• Fibronectin
• Hepatocyte
• Keratin filament

• Cell proliferation
• Liver carcinoma in situ
• Toxin binding

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35563

Doi: https://doi.org/10.6075/J0Q52NKX

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35563

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2023-08-17