Allen, Richard (2021). CIL:12109, Colpoda cucullus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NG4PS8

An image of a dikinetid in cross section near its proximal end. A postciliary ribbon and a transverse ribbon are seen. A short kinetodesmal fiber buds from the side of the kinetid. Coated vesicles or pits are located nearby that are part of the endosomal system. TEM taken on 6/3/69 by R. Allen with Philips 300 operating at 60kV. Bar = 0.5µm. The negative was printed to paper and the image was scanned with a flatbed scanner and 0.5µm bar added in Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:2861) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections were prepared at approximately 75nm thickness. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 3327

• Colpoda cucullus

• Axoneme
• Cartwheel complex
• Cell by organism
• Cell cortex
• Ciliated Protist
• Cilium
• Dikinetid
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule basal body

• Cambridge Culture Collection, Cambridge, England # LB1615/1
• Cell motility
• Cortical cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12109

Doi: https://doi.org/10.6075/J0NG4PS8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12109

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12