Allen, Richard (2021). CIL:12318, Halteria grandinella, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0HQ3Z1H

Halteria grandinella is a fresh water oligotrich ciliate. It lacks long rows of somatic cilia called kineties but has a few somatic ciliary bristles as well as a well developed oral and adoral or perioral ciliature. This section is a part of a serial series and the sections are thicker than normal so the EMs will appear less sharp when enlarged. For a more complete study of this cell refer to Grain, Protistologica 8:179-197, 1972. This figure is of a section near the cell's left adoral surface where the contractile vacuole is located and where some of the oral ciliature are exposed in longitudinal section. A surface indentation may be near the CV pore. One membranelle of the perioral ciliature is sectioned through its basal bodies. The cytosol contains condensed as well as expanded mitochondria. The uniformly round and electron transparent structures seen here appear filled with dense material under different fixation and staining conditions. These may be pigment granules. TEM taken on 3/12/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 3,800X. Bar = 2µm. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Thicker microtome sections prepared for a serial series. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print scanned for Photoshop
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 3472

• Halteria grandinella

• Cell by organism
• Ciliated Protist
• Contractile vacuole
• Eukaryotic cell
• Eukaryotic Protist
• Oral apparatus

• Carolina Biological Supply Co., NC, U.S.A.
• Contractile vacuole organization
• Cytoplasm organization
• Oral apparatus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12318

Doi: https://doi.org/10.6075/J0HQ3Z1H

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12318

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12