Allen, Richard (2021). CIL:36271, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00P0XN1

V. convallaria preserved in collidine buffered fixative (not cacodylate) has a less dense cytoplasm in which oranelles are easier to recognize. The rough ER has vesiculated but this vesiculation enhances the identification of loci where ER remains bound to the myonemes. These loci on the ER are called ‚Äúlinkage complexes." These complexes are unique and have not been reported outside the peritrichs. Macronucleus with nucleolus and cytopharynx at its distal end. TEM taken on 3/30/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,750X. Bar = 0.5µm. Published and adapted with permission from J. Cell Biol. 56:559-579, 1973. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39461) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 10367

• Vorticella convallaria

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cytopharynx
• Endoplasmic reticulum
• Eukaryotic cell
• Eukaryotic Protist
• Macronucleus

• Cambridge Culture Collection, Cambridge, England, # LB1690/1
• Cortical cytoskeleton organization
• Digestive system process
• Endoplasmic reticulum organization
• Macronucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36271

Doi: https://doi.org/10.6075/J00P0XN1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36271

Reference

• Allen RD. Structures linking the myonemes, endoplasmic reticulum, and surface membranes in the contractile ciliate Vorticella. J Cell Biol. 1973 Feb;56(2):559-79. https://doi.org/ 10.1083/jcb.56.2.559

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12