CIL:25303, Drosophila melanogaster
- Cite This Work
Maresca, Thomas; Salmon, Edward (2021). CIL:25303, Drosophila melanogaster. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QJ7G2T
Movie of colchicine-induced depolymerization of the metaphase spindle in a Drosophila S2 cell expressing GFP-tubulin.
Images were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Supplemental Video 3 in Maresca and Salmon, 2009 J Cell Biol 184:373-281.
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- Technical Details
Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Green fluorescent proteins from Aequorea
Processing history: unprocessed raw data
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- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25303
- Maresca and Salmon, 2009 J Cell Biol 184:373-281 https://www.ncbi.nlm.nih.gov/pubmed/?term=19193623
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