Fawcett, Don W.; Schliwa, Manfred; Osborn, Mary; Weber, Klaus (2021). CIL:36043, Pterophyllum scalare, pigment cell (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0J67FRC

Figures 404 (upper panels A, B), 405 (middle) and 406 (lower) from Chapter 16 (Cytoplasmic matrix and cytoskeleton) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Microtubules and pigment granule transport. Upper panels compare dispersed (A) vs. aggregated (B) pigment granule observed using interference microscopy in living fish melanophores. Arrows in B show that the outer boundary of the cell remains unchanged. Middle panel shows radially oriented microtubules in a melanophore with dispersed pigment, revealed by antibody staining of tubulin with FITC-conjugated secondary. In cells with aggregated pigment, the radial disposition remains unchanged, although in the region of concentrated pigments, only a few fibers can be observed (lower panel). Figures from Schliwa, Osborn and Weber, J. Cell Biol. 76:229-236, 1978 (PMID:338618). A PDF copy of the accompanying chapter is available on the ASCB’s BioEDUCATE website.

• 2021

• Fawcett, Don W.
• Osborn, Mary
• Schliwa, Manfred
• Weber, Klaus

Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: differential interference contrast microscopy; fluorescence microscopy
Parameter imaged: optical path length gradient; fluorescence emission
Source of contrast: boundaries between regions with different refractive index; distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody
Data qualification: Processed

• Cell Image Library Group ID: 9915

• Pterophyllum scalare

• Microtubule
• Pigment cell (sensu Vertebrata)
• Pigment granule

• Microtubule-based transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36043

Doi: https://doi.org/10.6075/J0J67FRC

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36043

Other resource

• Fawcett, Don W. "The Cell." 2nd ed., W.B. Saunders, 1981, ISBN 0721635849. https://www.ascb.org/career-development/teaching/don-fawcetts-the-cell/

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2022-08-12