CIL:39731, Gallus gallus gallus, erythrocyte
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- Cite This Work
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Bednar, Jan; Horowitz, Rachel; Grigoryev, Sergei; Carruthers, Lenny; Hansen, Jeffrey; Koster, Abraham J.; Woodcock, Christopher (2021). CIL:39731, Gallus gallus gallus, erythrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0R78D0P
- Description
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TEM image of frozen hydrated soluble chromatin isolated from chicken erythrocyte nuclei and vitrified in low salt. Polynucleosomes adopt an irregular zigzag fiber conformation (a,b,k), and show a 'stem' motif. Similar features are seen in reconstituted polynucleosomes (c,d,f-i). However, if reconstitutes lack linker histone H5, the stem conformation is absent (c).
See Fig 1 in J Bednar et al. 1998 Nucleosomes, linker DNA and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatin. Proc Natl Acad Sci USA 95:14173-14178. - Date Issued
- 2021
- Researchers
- Methods
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Isolated nuclei were prepared from chicken erythrocyte nuclei and polynucleosomes released after treatment with micrococcal nuclease. Reconstituted polynucleosomes were prepared by salt dialysis using purified DNA and histones. Samples were fixed with 0.1% glutaraldehyde for 4 hr at 4C, placed on holey carbon films, vitrified by plunge freezing, and observed in the frozen hydrated state using a Philips CM10 TEM operated at 60KV. Low dose images were recorded on Kodak SO163 film.
See also: J Bednar et al. 1998 Nucleosomes, linker DNA and linker histone form a un ique structural motif that directs the higher-order folding and compaction of chromatin. Proc Natl Acad Sci USA 95:14173-14178. - Technical Details
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Preparation: glutaraldehyde fixed tissue; cryofixed tissue
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution
Visualization methods: visualization of contiguous regions
Data qualification: Processed - Series
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- Identifier
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Samplenumber: 39731
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39731
- J Bednar et al. 1998 Nucleosomes, linker DNA and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatin. Proc Natl Acad Sci USA 95:14173-14178. https://www.ncbi.nlm.nih.gov/pubmed/?term=9826673
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
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- UC Regents
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12