Microscopy product ID: 27
2D Image
Description | Single tilt image (zero degree tilt) through a 4 um thick section of spiny dendrite from a medium spiny neuron that was injected with Lucifer Yellow then photooxidized |
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Description | tar file contains the original tilt images compressed using the compress command; osaka4.????.f.Z. Unfortunately; the original fiducial mark file was not saved. |
Animation
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Description | Aligned and cropped tilt images from a striatal medium spiny neuron that was injected with Lucifer Yellow and then photooxidized. No fiducial marks were available and so the alignment and subsequent reconstruction are poor. The quality of the microscopic images is fine though, so they can be re-aligned. |
Segmentation
Description | manual segmentation using Xvoxtrace; surfaced with Synu using Nuage algorithm; tarfile contains all synu objects listed; the associated Viewdata file required by Synu for viewing; and Xvoxtrace file (osaka4.trace) used to contour the objects. |
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Reconstruction
Description | Tomographic reconstruction of a portion of medium spiny neuron in a 4 um thick section. This data set is poorly aligned due to a lack of adquate fiducial markers. This volume is an example of a poor reconstruction |
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Description | Volume reconstruction of osaka4 in Analyze format; volume has been sigma filtered using Analyze |
Full metadata
- Collection
- Cite This Work
-
Martone, Maryann; Yamada, Naoko; Arbuthnott, Gordun; Ingham, Cali; Young, Stephen J. (2017). Microscopy product ID: 27. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0NG4QC9
- Creation Date
- Project: 1992-01-01.
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Product type: SINGLE TILT. Microscopy type: UHVEM. Instrument: Hitachi UHVEM
Strain: Sprague Dawley
- Funding
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NIH
- Series
- Scientific Name
- Anatomy
- Topic
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View formats within this collection
- Language
- No linguistic content; Not applicable
- Related Resource
- Microscopy product 27 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB27
Other version
- License
-
Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
-
Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22