Allen, Richard (2021). CIL:38828, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02R3R0W

High resolution overview of Coleps hirtus cell seen in this median transverse section. This barrel-shaped cell has a few rows of cilia and the cell is covered with calcified polysaccharide platelets that are housed within the alveoli. In this TEM the platelets are absent as they dissolved during preparation for electron microscopy. The macronucleus, a food vacuole, and a large empty vacuole is evident. Typical ciliate cytoplasm is present. TEM taken on 5/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 3,120X.

• 2021

• Allen, Richard

The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: film; Print from negative scanned for Photoshop.
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 9790

• Coleps hirtus

• Alveolar membranes
• Cellular_component
• Ciliated Protist
• Contractile vacuole
• Eukaryotic cell
• Eukaryotic Protist
• Kinetal rows of cilia
• Macronucleus
• Plasma membrane

• Calcium deposition
• Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
• Ciliary or flagellar motility
• Detection of calcium ion

• data
• image

• No linguistic content; Not applicable

Samplenumber: 38828

Doi: https://doi.org/10.6075/J02R3R0W

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL38828

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2025-01-29