LaFountain, James R.; Oldenbourg, Rudolf (2021). CIL:10725, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FN15DV

Movie of anaphase A and B of meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis.
Time-lapse polarization microscopy using a Nikon
Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 30 sec over 60 min using a QImaging Retigo EXi CCD camera. Raw images were processed
using 4-frame algorithm (Shribak and Oldenbourg, 2003). The time series of images on which the movie is based is included in this grouped set.

• 2021

• LaFountain, James R.
• Oldenbourg, Rudolf

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD); Processed image based on 4 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 4-frame algorithm

• Cell Image Library Group ID: 11200

• Nephrotoma
• Nephrotoma suturalis

• Nuclear chromosome
• Primary spermatocyte
• Spindle

• Male meiosis chromosome segregation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10725

Doi: https://doi.org/10.6075/J0FN15DV

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10725

Reference

• LaFountain JR, Oldenbourg R (2004) Mol Biol Cell 15, 5346-5355. https://doi.org/10.1091/mbc.e04-06-0524
• Oldenbourg R, Mei G (1995) J Microsc 180, 140-147. https://doi.org/10.1111/j.1365-2818.1995.tb03669.x
• Shriback M, Oldenbourg R (2003) Appl Opt 42, 3009-3017. https://doi.org/10.1364/AO.42.003009

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12