Lauzon, Dee; Lancelle, Sue; Rice, Marian (2021). CIL:39780, Mus musculus, myofibril. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06M365C

Transmission electron micrograph of ventricle tissue of a mouse cardiac muscle. This image shows the various areas of a muscle fiber. The Z lines are the very dark borders that link sarcomeres. The area between the Z lines is further divided into two lighter colored bands at either end called the I-bands, and a darker, grayish band in the middle called the A-band. The A band appears darker than the I band. The H zone is the bright region in the A band where there is no overlapping actin and myosin when the muscle is in the relaxed state. There are also many darkly stained mitochondria in this image.

• 2021

• Lancelle, Sue
• Lauzon, Dee
• Rice, Marian

Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr's resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.

Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment; ethanol dehydration
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10969

• Mus musculus

• A band
• H zone
• I band
• Myofibril
• TS25,ventricle
• Z disc

• Muscle contraction

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39780

Doi: https://doi.org/10.6075/J06M365C

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39780

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2022-08-12