Allen, Richard (2021). CIL:36754, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09W0D5D

Acid phosphatase is present in tubules retrieved from late DV-III. The tubules and rounded expansions arising as a result of late DV-III membrane retrieval contain an electron-opaque reaction product when the cells are incubated in Gomori‚Äôs reagent and lead salts. This indicates that acid phosphatase (AcPase) is being retrieved from the late phagolysosomes before the spent vacuole contents are released at the cytoproct. In fact, spent vacuoles no longer contain active AcPase and are free of AcPase-reaction product before they are defecated. (For details of lysosome recycling see Allen and Fok, J. Cell Biol. 99:1955-1959, 1984). TEM taken on 4/27/84 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm. Part published in J. Cell Biol. 99:1955-1959, 1984. Adapted with permission.

• 2021

• Allen, Richard

Glutaraldehyde fixation followed by Gomori staining, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This image is suitable for qualitative analysis. A high resolution version of this image in the library (CIL:40566) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 11153

• Paramecium multimicronucleatum

• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Phagolysosome

• Digestive system process

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36754

Doi: https://doi.org/10.6075/J09W0D5D

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36754

Reference

• J Cell Biol. 1984 Dec;99(6):1955-9. https://www.ncbi.nlm.nih.gov/pubmed/?term=6501410

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2022-08-12