Hotta, Azusa; Kawakatsu, Tomomi; Nakatani, Tomoya; Sato, Toshitaka; Matsui, Chiyuki; Sukezane, Taiko; Akagi, Tsuyoshi; Hamaji, Tomoki; Grigoriev, Ilya; Akhmanova, Anna; Takai, Yoshimi; Mimori-Kiyosue, Yuko (2021). CIL:12290, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0S75F25

To determine the extent to which extracellular matrix proteins colocalize with the microtubule-anchoring factor LL5s (LL5α), MCF-10A epithelial cells were immunostained with antibodies to laminin receptors α3 (green), α6 (blue) and LL5s (red). Analyses suggest that LL5s are colocalized with activated pools of integrins associated with laminin-5. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file for Fig. 3 Panel D-c from J Cell Biol (2010) 189 (5):901-917.

• 2021

• Akagi, Tsuyoshi
• Akhmanova, Anna
• Grigoriev, Ilya
• Hamaji, Tomoki
• Hotta, Azusa
• Kawakatsu, Tomomi
• Matsui, Chiyuki
• Mimori-Kiyosue, Yuko
• Nakatani, Tomoya
• Sato, Toshitaka
• Sukezane, Taiko
• Takai, Yoshimi

MCF-10A epithelial cells were fixed with 1:1 mixture of methanol/acetone (-20° C, 10 min), permeabilized with 0.5% Triton in PBS, and immunostained sequentially with primary, then secondary antibodies and laminin receptor (Cy5, 633 nm laser), laminin receptor α6 (Cy2, 488 nm laser) and laminin receptor α3(rhodamine red-X, 543 nm laser), and observed using a confocal laser-scanning microscope system. For additional details see: J. Cell Biol. 2010. 189(5):901-917

Preparation: methanol fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Cy2; Cy5; X-Rhodamine
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 8194

• Homo sapiens

• Cell cortex part
• Epithelial cell
• Microtubule

• Cell-substrate adhesion
• Epithelial cell migration
• Establishment or maintenance of apical/basal cell polarity
• MCF 10A

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12290

Doi: https://doi.org/10.6075/J0S75F25

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12290

Reference

• Hotta A, Kawakatsu T, Nakatani T, Sato T, Matsui C, Sukezane T, Akagi T, Hamaji T, Grigoriev I, Akhmanova A, Takai Y, Mimori-Kiyosue Y. Laminin-based cell adhesion anchors microtubule plus ends to the epithelial cell basal cortex through LL5alpha/beta. J Cell Biol. 2010 May 31;189(5):901-17. https://doi.org/10.1083/jcb.200910095
• J. Cell Biol. 2010. 189(5):901-917 https://www.ncbi.nlm.nih.gov/pubmed/?term=0513769

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12