Microscopy product ID: 6659
2D Image
Description | Single tilt image of a 250 um thick section of a Drosophila S2 cell infected with flock house virus (FHV-1) imaged with intermediate voltage electron microscopy, taken at zero degree tilt. The dark specks are colloidal gold particles that have been applied to the surfaces of the section to serve as fiducial marks for subsequent alignment. |
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Description | Zip file containing the single axis tilt images (fhv2.st) in IMOD MRC format along with many of the ancillary files generated by iMOD. |
Segmentation
Description | Manual segmentation of mitochondrial membranes and replication centers (RC's) of FHV-1 using Xvoxtrace v2.13. Contours were sufaced using Synu. |
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Description | Zip file containing original trace files (FHVmito2.trace and FHVmitonew.trace), along with some of the surface files in Synu (*.synu) and open inventor (*.iv) format. It appears that only some of the surface renderings for the rc objects are available; the rest will have to be regenerated; no surfaced objects associated with mitochondrial membranes were submitted. An animation of the segmented mitochondrion and the viral spherules in Quicktime format is also included (6659_seg.mov). Please note that Jinx (http://ncmir.ucsd.edu/downloads/jinx.shtm) can be used to read traces generated with Xvoxtrace. Jinx requires the original reconstruction file, available from the "Reconstruction' download page. |
Reconstruction
Description | Single computed slice through a tomographic reconstruction of mitochondria from Drosophila S2 cells infected with flock house virus (FHV-1). To see the relationship between the 2D tilt image and the reconstructed area, please view the image map. Note that the reconstruction (inset on image map) is flipped vertically relative to the original tilt data. |
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Description | Zip file (FHV2_vol.zip) containing a tomographic reconstruction of mitochondria from Drosophila S2 cells infected with flock house virus (FHV-1). Both the .img and .hdr files are included (fhv2.sub.img/hdr). |
Animation
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Description | Animation through the computed slices of a tomographic reconstruction of mitochondria from Drosophila S2 cells infected with flock house virus (FHV-1). |
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- Cite This Work
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Perkins, Guy A.; Ahlquist, Paul; Ellisman, Mark H.; Kopek, Benjamin G. (2017). Microscopy product ID: 6659. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0542NFT
- Creation Date
- Project: 2003-06-01.
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEM-4000EX IVEM
- Funding
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National Institutes of Health
- Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Related Resources
- Kopek BG, Perkins G, Miller DJ, Ellisman MH, Ahlquist P (2007). Three-dimensional analysis of a viral RNA replication complex reveals a virus-induced mini-organelle. PLoS Biol, 5(9):e220. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1945040/; PLoS Biology. https://doi.org/10.1371/journal.pbio.0050220
- Microscopy product 6659 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB6659
Primary associated publication
Other version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22