Allen, Richard (2021). CIL:36231, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WH2NXD

Cytosolic pressure may be one force used to expel the undigested contents from spent vacuoles, however, tubulation and retrieval of the spent vacuole membrane will ultimately eliminate all of the vacuole contents as well as the spent vacuole membrane and allow the cytoproct lips to be pulled back together. The vesiculated vacuole membrane can then be recycled. TEM taken on 4/21/78 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,250X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Tetrahymena pyriformis

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cytoproct
• Eukaryotic cell
• Eukaryotic Protist
• Plasma membrane

• Defecation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36231

Doi: https://doi.org/10.6075/J0WH2NXD

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36231

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2022-08-12