Ris, Hans (2021). CIL:25331, Drosophila melanogaster, salivary gland. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06H4G9T

Salivary glands from 3rd instar larvae of Drosophila melanogaster were dissected, fixed with formalin and stained with uranyl acetate, embedded in epoxy resin, and 0.5 micrometer sections prepared (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV.

Image shows a series of bands and interbands in a polytene chromosome. This image was taken with a tilt angle of 45 degrees. The accompanying grouped image, taken at 55 degrees forms a stereo pair, providing an oblique 3D view of the chromosome.

• 2021

• Ris, Hans

Preparation: formaldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 6832

• Drosophila melanogaster

• Polytene chromosome
• Salivary gland

• data
• image

• No linguistic content; Not applicable

Samplenumber: 25331

Doi: https://doi.org/10.6075/J06H4G9T

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25331

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2022-08-12