Antipa, Gregory (2021). CIL:24921, uncultured Scuticociliatia, Ancistrum sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VH5MZ8

Ancistrum is a marine ciliate that inhabits the mantle cavity of mytiliid mussels. This image shows a good view of the macronucleus, mitochondria with tubular cristae, and what are probably flask-shaped endosomes. This group of images is located in a part of the cytoplasm that especially contains these vesicles, and this image has a view at an appropriate angle to exhibit what may be dynamin or a dynamin-like protein decorating the tube of the flask shaped organelle. In addition, the macronuclear membrane is well demonstrated. TEM taken by G. Antipa in 1969 on a Hitachi HU11A operating at 75kV. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 60nm thickness.

• 2021

• Antipa, Gregory

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 11095

• Ancistrum sp.
• Uncultured Scuticociliatia

• Cell by organism
• Ciliated Protist
• Cytoplasm
• Endosome
• Eukaryotic cell
• Eukaryotic Protist
• Macronucleus
• Mitochondrial crista
• Mitochondrial envelope
• Mitochondrion
• Peroxisome

• Cytoplasm organization
• Endosome localization
• Macronucleus organization
• Mitochondrion organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24921

Doi: https://doi.org/10.6075/J0VH5MZ8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24921

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2022-08-12