Caro, Lucien (2021). CIL:37122, Cavia porcellus, exocrine cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W37TZP

Electron microscope autoradiograph showing radiographic leucine over elements of the rough endoplasmic reticulum. A few grains are also found over small, smooth surfaced vesicles of the Golgi complex. Image corresponds to part of Figure 3 in J. Cell Biol. 20:473-495. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

• 2021

• Caro, Lucien

Anesthetized animals were intravenously injected with 1-5 millicuris of DL-leucine 4,5-H^3 with a specific activity of 3570 mc/mM. At times ranging from 4 min to 15 hrs after injection the pancreas was removed and fixed in osmium tetroxide. Tissue was embedded in methacrylate, sectioned, and post-stained with uranyl acetate.

Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification x21,000.

Preparation: osmium tetroxide fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); electron microscope autoradiograph
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; metabolically incorporated radioisotope
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10844

• Cavia porcellus

• Exocrine cell
• Golgi-associated vesicle
• Rough endoplasmic reticulum

• Protein secretion
• Protein transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 37122

Doi: https://doi.org/10.6075/J0W37TZP

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL37122

Reference

• J Cell Biol. 1964 Mar;20:473-95 https://www.ncbi.nlm.nih.gov/pubmed/?term=14128049

Other resource

• George E. Palade EM Slide Collection: https://library.medicine.yale.edu/find/palade

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2022-08-12