Microscopy product ID: 6674
Reconstruction
Description | Wide field mosaic of mouse hippocampus immunolabeled for pannexin 1 (green), GFAP (blue) and connexin 43 (red). The section was also counterstained with DAPI (not shown). |
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Description | 176 tiles (16x11) were digitally stitched together to generate a 3D mosaic montage of the mouse hippocampus labeled with three different antibodies (Green = Pannexin1 protein, Red = Connexin43 protein, Blue = GFAP protein within astrocytes). This zipped tar file contains 24 reconstructed mosaic images (file names labeled 00-23), one for each of the 24 optical Z-sections that were acquired with a step distance of 0.5 microns. Additionally, the zip file contains a mosaic 2D maximum intensity projection of all 24 Z-sections (file name containing "proj"). |
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Description | This image is a mosaic 2D maximum intensity projection of all 24 Z-sections. Voxel scale for this flattened projection of the mosaic hippocampus is 0.41 x 0.41 x 11.5 microns/pixel. The tiles in this image have been normalized, but the colors in the resulting image have not been adjusted. |
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- Cite This Work
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Cone, Angela; Sosinsky, Gina; Martone, Maryann (2017). Microscopy product ID: 6674. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0N29WSH
- Creation Date
- Microscopy product: 2009-01-23. Experiment: 2008-12-22. Project: 2008-11-06.
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Product type: MOSAIC. Microscopy type: LASER SCANNING CONFOCAL. Instrument: Olympus Fluoview 1000
- Series
- Scientific Name
- Anatomy
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- Language
- No linguistic content; Not applicable
- Related Resource
- Microscopy product 6674 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB6674
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- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22