Woodcock, Christopher (2021). CIL:502, Patiria miniata, sperm. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0C24VPC

Echinoderm sperm nuclei are unusual in that chromatin fibers are clearly seen, allowing their diameter to be accurately determined. Here, sperm from the starfish P. miniata were treated to remove the outer membrane, cryo-fixed by plunging into liquid ethane, freeze-substituted in acetone and low-temperature embedded in Lowicryl K11M. Thin sections were stained with uranyl acetate, and examined using a FEI CM10 TEM operated at 100KV. This image shows the cup-shaped nucleus surrounding the acrosome. Flagellar axonemes are also present. Chromatin fibers in the nucleus are clearly visualized.

• 2021

• Woodcock, Christopher

Preparation: cryofixed tissue; tissue in plastic embedment; freeze substitution
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image; film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10230

• Patiria miniata

• Male germ cell nucleus
• Sperm

• Nucleus organization
• Reproduction
• Structural constituent of chromatin

• data
• image

• No linguistic content; Not applicable

Samplenumber: 502

Doi: https://doi.org/10.6075/J0C24VPC

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL502

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=8138565

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2022-08-12