Allen, Richard (2021). CIL:38689, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01G0KMP

A high resolution view of the radial arm of the Contractile Vacuole Complex or CVC each contain a collecting canal enclosed by a microtubular ribbon split lengthwise into several smaller ribbons, a meshwork of smooth spongiome and, peripheral to this, the bundles of tubules of the decorated spongiome. The microtubules along the collecting canal are immunogold-labeled with anti-b-tubulin as are some single microtubules in the area. TEM taken on 7/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 7,900X.

• 2021

• Allen, Richard

Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The raw film was scanned with a Nikon Coolscan 9000ED. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 9640

• Paramecium tetraurelia

• Cell by organism
• Ciliated Protist
• Contractile vacuolar membrane
• Contractile vacuole
• Cytoplasmic microtubule
• Eukaryotic cell
• Eukaryotic Protist

• Contractile vacuole organization
• Cytoplasm organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 38689

Doi: https://doi.org/10.6075/J01G0KMP

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL38689

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12