Lam, Wai Yan; Rice, Marian (2021). CIL:27227, Fundulus heteroclitus, epidermal cell, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00Z7268

Low magnification of the epidermal region of the Fundulus heteroclitus scale. The epidermis is separated from the collagen layers of the dermis by the basement membrane. The cells of the epidermis (top) are juxtaposed and well adhered to one another. The cells in the dermis, called fibroblasts, which synthesize extracellular matrix and collagen, are much more elongated and appear to be traveling through the collagen fibrils. Present in the epidermal cells are clusters of dark needle-like crystals that appear to be mineral deposits. It is important to note that the nuclear membranes of the fibroblasts appear to be dilated and lifted from the chromatin. This may have been an artifact of poor fixation.

• 2021

• Lam, Wai Yan
• Rice, Marian

Fundulus heteroclitus scales were chemically fixed with 2.5% glutaraldehyde, 2% formaldehyde in 0.1M cacodylate buffer (pH 7.3), then post-fixed in 4% osmium tetroxide and stained en bloc in 1% uranyl acetate. The scales were then dehydrated in a graded series of ethanol and infiltrated with Spurr's resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph was imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80 kV.

Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; dehydrated in ethanol
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9597

• Fundulus heteroclitus

• Basement membrane
• Collagen
• Epidermal cell
• Fibroblast
• Mineral deposits

• Cytoskeleton organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 27227

Doi: https://doi.org/10.6075/J00Z7268

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL27227

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2022-08-12