Price, Diana; Laakso, Aki; Cyr, Michele; Martone, Maryann; Yamada, Naoko; Thor, Andrea; Berlanga, Monica (2021). CIL:39992, Mus musculus, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0736R1G

A single computed slice from a tomographic reconstruction of a lucifer yellow injected spiny dendrite from a striatal medium spiny neuron from the neostriatum of a dopamine transporter knock out mouse. This reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.

• 2021

• Berlanga, Monica
• Cyr, Michele
• Laakso, Aki
• Martone, Maryann
• Price, Diana
• Thor, Andrea
• Yamada, Naoko

Tissue from mouse strain: B6;129-/Slc6a3 tm2Mca, dopamine transporter knockout. Individual cells in fixed tissue were injected with Lucifer yellow and stored in 4% paraformaldehyde at 4° C. Fluorescence was photoconverted using DAB and a confocal laser light source. After this process, tissue was washed thoroughly in PBS and processed for EM by osmication, dehydration, and embedding in epoxy resin. For detailed methods, see the protocol provided in the CCDB. The tomogram was generated using an Hitachi UHVEM. Single tilt images spanned -70 to 70° in 2° increments. Magnification, 3000X, accelerating voltage, 3.0 MeV.

Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue; tissue in epoxy resin embedment; osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: detection of electrons; illumination by electrons
Parameter imaged: electron density
Visualization methods: intracellular filling; lucifer yellow
Data qualification: Processed

• Cell Image Library Group ID: 17308

• Mus musculus

• CNS neuron (sensu Vertebrata)
• Dendrite
• Dendritic spine

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39992

Doi: https://doi.org/10.6075/J0736R1G

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39992

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0NC610T

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2022-08-12