Ellisman, Mark; Larson, Stephen; Maynard, Sarah; Martone, Maryann; Bushong, Eric (2021). CIL:40234, Mus musculus, CNS neuron (sensu Vertebrata), glial cell, blood vessel endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0N58MH8

Coronal section of rat brain triple-labeled with fluoromyelin (green), DiI (red), and TO-PRO3 (blue) which stain for myelin, blood vessels, and cell bodies, respectively. A maximum projection image was compiled by acquiring images across the entire rat brain section, and through 16 optical sections spanning the thickness of the section. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. It is also available through the Whole Brain Catalog (http://wholebraincatalog.org).

• 2021

• Bushong, Eric
• Ellisman, Mark
• Larson, Stephen
• Martone, Maryann
• Maynard, Sarah

The animal was perfused with Ringer's solution for 2 minutes followed by DiI for 10 minutes, and then 2% paraformaldehyde for 10 minutes. The brain was post-fixed overnight, and the tissue subsequently sectioned. After buffer washes, sections were stained with Fluoromyelin (1:100 overnight in cold room), rinsed with buffer washes, and subsequently stained TO-PRO3 (1:3000) for 45 minutes at room temp, rinsed and mounted using Gelvatol. 16 z-planes at 5 µm intervals across the entire section used to generate the tiled 3-D mosaic, acquired with an Olympus Fluoview 1000.

Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; compartmentalization of stain or label
Visualization methods: DiI; fluorescent labels
Data qualification: Processed

• Cell Image Library Group ID: 11116

• Mus musculus

• Blood vessel endothelial cell
• CNS neuron (sensu Vertebrata)
• Glial cell
• Myelin sheath
• Nucleus

• Central nervous system structural organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40234

Doi: https://doi.org/10.6075/J0N58MH8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40234

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J03X86F1

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2022-08-12