Maddox, Paul S.; Hyndman, Francie; Monen, Joost; Oegema, Karen; Desai, Arshad (2021). CIL:28778, Caenorhabditis elegans, embryo. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J08K77R6

Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 in strain OD31. As seen for GFP-CeCENP-A (CIL 28780), discrete foci of the GFP signal within the nucleus coalesce to form holocentric kinetochore plates during prophase. GFP image on left and DIC image on right. After nuclear envelope breakdown, chromosomes appear as paired lines and congress to the metaphase plate. At metaphase, sister kinetochores form two lines facing opposite spindle poles. During anaphase, foci of GFP-KNL-2 were seen to form in the spindle area. The video is ∼16 min long, and the frame is ∼30 µm top to bottom. DIC is shown on the right, and GFP is shown on the left. Images were acquired at 10-s intervals.

• 2021

• Desai, Arshad
• Hyndman, Francie
• Maddox, Paul S.
• Monen, Joost
• Oegema, Karen

Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP–γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD camera (Orca ER; Hamamatsu) binning 2 × 2. Strain OD31 expressing GFP–KNL-2 was imaged in the same manner without the 1.5× auxiliary magnification.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy; differential interference contrast microscopy
Parameter imaged: fluorescence emission; optical path length gradient
Source of contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization methods: EGFP; visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11164

• Caenorhabditis elegans

• Embryo
• KNL-2

• Anaphase
• Metaphase
• Mitosis
• Mitotic nuclear envelope disassembly
• Prophase

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 28778

Doi: https://doi.org/10.6075/J08K77R6

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL28778

Reference

• J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5. https://www.ncbi.nlm.nih.gov/pubmed/?term=17339379

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12