Hu, Chi-Kuo (2021). CIL:7224, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VX0FJ2

Distribution of Golgi Apparatus during cell division. Hela cells fixed with MeOH on ice for 3 min, and stained with a primary antibody against the Golgi and FITC conjugated DM1a antibody (green) against microtubules. Secondary antibody was Alexa 594 for the Golgi (red). A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected with an Orca ER CCD camera.
Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, and 568 Laser/ATOF 605/52.

• 2021

• Hu, Chi-Kuo

Preparation: methanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 594; Fluorescein (FITC); labeled primary antibody
Processing history: unprocessed raw data

• Cell Image Library Group ID: 2889

• Homo sapiens

• Epithelial cell
• Golgi apparatus
• Microtubule

• Cell division
• HeLa

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7224

Doi: https://doi.org/10.6075/J0VX0FJ2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7224

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2022-08-12