CIL:40670, Drosophila melanogaster
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- Collection
- Cite This Work
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Gelfand, Vladimir; Larsen, Adam (2021). CIL:40670, Drosophila melanogaster. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00G3J1G
- Description
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Time lapse movie prepared from fluorescence microscopy time series of a Drosophila S2 cell stably expressing mCherry-alpha-tubulin and spreading on a ConA-coated substrate in the presence of cyto D. Long processes containing microtubule bundles are formed. Microtubule looping and entry into the bundles appears to contribute to the growth of the processes.
This movie is part of a grouped set that includes the corresponding time series stack of images. - Date Issued
- 2021
- Researchers
- Methods
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Time series of widefield fluorescence images were recorded using a Nikon U-2000 inverted microscope equipped with a Nikon 100x NA 1.49 objective lens, Nikon perfect Focus system and Cascade II EMCCD camera (Roper Scientific)driven by Metamorph software. See also Fig 3C and Supplemental Movie S8 in A.L. Jolly et al. 2010 Proc Natl Acad Sci USA 107:12151-12156.
- Technical Details
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Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: electron multiplying CCD (EMCCD)
Imaging mode: widefield illumination; fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: mCherryFP - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40670
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40670
- A.L. Jolly et al. 2010 Proc Natl Acad Sci USA 107:12151-12156 https://www.ncbi.nlm.nih.gov/pubmed/?term=20566873
- Amber L. Jolly, Hwajin Kim, Divya Srinivasan, Margot Lakonishok, Adam G. Larson, and Vladimir I. Gelfand (2010). Kinesin-1 heavy chain mediates microtubule sliding to drive changes in cell shape. PNAS. https://doi.org/10.1073/pnas.1004736107
- Morris E Feldman,Beth Apsel,Aino Uotila,Robbie Loewith,Zachary A Knight,Davide Ruggero,Kevan M Shokat (2009). Active-Site Inhibitors of mTOR Target Rapamycin-Resistant Outputs of mTORC1 and mTORC2. https://doi.org/10.1371/journal.pbio.1000038
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Reference
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12