Gelfand, Vladimir; Larsen, Adam (2021). CIL:40670, Drosophila melanogaster. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00G3J1G

Time lapse movie prepared from fluorescence microscopy time series of a Drosophila S2 cell stably expressing mCherry-alpha-tubulin and spreading on a ConA-coated substrate in the presence of cyto D. Long processes containing microtubule bundles are formed. Microtubule looping and entry into the bundles appears to contribute to the growth of the processes.
This movie is part of a grouped set that includes the corresponding time series stack of images.

• 2021

• Gelfand, Vladimir
• Larsen, Adam

Time series of widefield fluorescence images were recorded using a Nikon U-2000 inverted microscope equipped with a Nikon 100x NA 1.49 objective lens, Nikon perfect Focus system and Cascade II EMCCD camera (Roper Scientific)driven by Metamorph software. See also Fig 3C and Supplemental Movie S8 in A.L. Jolly et al. 2010 Proc Natl Acad Sci USA 107:12151-12156.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: electron multiplying CCD (EMCCD)
Imaging mode: widefield illumination; fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: mCherryFP

• Cell Image Library Group ID: 11370

• Drosophila melanogaster

• Cytoskeleton
• Microtubule

• Cytoskeleton organization
• S2

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 40670

Doi: https://doi.org/10.6075/J00G3J1G

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40670

Reference

• A.L. Jolly et al. 2010 Proc Natl Acad Sci USA 107:12151-12156 https://www.ncbi.nlm.nih.gov/pubmed/?term=20566873
• Amber L. Jolly, Hwajin Kim, Divya Srinivasan, Margot Lakonishok, Adam G. Larson, and Vladimir I. Gelfand (2010). Kinesin-1 heavy chain mediates microtubule sliding to drive changes in cell shape. PNAS. https://doi.org/10.1073/pnas.1004736107
• Morris E Feldman,Beth Apsel,Aino Uotila,Robbie Loewith,Zachary A Knight,Davide Ruggero,Kevan M Shokat (2009). Active-Site Inhibitors of mTOR Target Rapamycin-Resistant Outputs of mTORC1 and mTORC2. https://doi.org/10.1371/journal.pbio.1000038

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2022-08-12