Morselli, Eugenia; Mariño, Guillermo; Bennetzen, Martin v.; Eisenberg, Tobias; Megalou, Evgenia; Schroeder, Sabrina; Cabrera, Sandra; Bénit, Paule; Rustin, Pierre; Criollo, Alfredo; Kepp, Oliver; Galluzzi, Lorenzo; Shen, Shensi; Malik, Shoaib Ahmad; Maiuri, Maria Chiara; Horio, Yoshiyuki (2021). CIL:13898, Saccharomyces cerevisiae S288c. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0KD1WM2

Saccharomyces cerevisiae (WT BY4741-MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0), transformed with EGFP-Atg8p, undergoing chronological aging on small synthetic 2% glucose media (SMD) with supplementation of 4 mM spermidine. Spermidine was added to stationary cultures at day 1 of the aging experiments. Image represents yeast morphology, visualized by DIC. DIC image was captured using a widefield microscope (Axioskop; Carl Zeiss, Inc) fitted with a Plan Neofluar 63× 1.25 NA oil immersion objective. Image acquired with a camera (SPOT 9.0 Monochrome 6; Diagnostic Instruments, Inc) using Metamorph software (Universal Imaging Corp) and processed with IrfanView and Photoshop (CS2; Adobe) software. Specifically, picture processing involved coloring and cropping of representative areas and was performed with IrfanView. In addition, linear adjustments of contrast and brightness were applied with Photoshop (using equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 2A, WT/Spd/DIC panel, in Morselli et al. J Cell Biol 192: 615-629

• 2021

• Bennetzen, Martin v.
• Bénit, Paule
• Cabrera, Sandra
• Criollo, Alfredo
• Eisenberg, Tobias
• Galluzzi, Lorenzo
• Horio, Yoshiyuki
• Kepp, Oliver
• Maiuri, Maria Chiara
• Malik, Shoaib Ahmad
• Mariño, Guillermo
• Megalou, Evgenia
• Morselli, Eugenia
• Rustin, Pierre
• Schroeder, Sabrina
• Shen, Shensi

Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Processing history: brightness and contrast adjusted
Data qualification: Processed

• Cell Image Library Group ID: 5406

• Saccharomyces cerevisiae S288c

• Autophagic vacuole

• Autophagy
• BY 4741

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13898

Doi: https://doi.org/10.6075/J0KD1WM2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13898

Reference

• J Cell Biol 192: 615-629. https://www.ncbi.nlm.nih.gov/pubmed/?term=21339330

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12