Ball, David; Peccoud, Jean (2021). CIL:35643, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09Z93VR

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc20, a cell-cycle regulated activator of anaphase-promoting complex/cyclosome (APC/C) required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; potential Cdc28p substrate novel protein thought to be involved in cell cycle regulation.These phase,gfp images are part of an image group that ranges from CIL:35640-35659. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

• 2021

• Ball, David
• Peccoud, Jean

The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Please see the microscopy section in the referenced manuscript for details for image analysis.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 10615

• Saccharomyces cerevisiae

• Anaphase-promoting complex
• CDC20 protein
• Mitotic checkpoint complex

• Activation of mitotic anaphase-promoting complex activity
• Mannoprotein catabolic process
• Mitotic metaphase/anaphase transition
• Mitotic sister chromatid segregation
• Mitotic spindle elongation
• Protein binding
• Ubiquitin-protein ligase activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35643

Doi: https://doi.org/10.6075/J09Z93VR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35643

Reference

• Ball DA, Marchand J, Poulet M, Baumann WT, Chen KC, Tyson JJ, Peccoud J. Oscillatory dynamics of cell cycle proteins in single yeast cells analyzed by imaging cytometry. PLoS One. 2011;6(10):e26272. https://doi.org/10.1371/journal.pone.0026272
• PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26. https://www.ncbi.nlm.nih.gov/pubmed/?term=22046265

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2025-01-30