Ip, Wallace (2021). CIL:4659, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02Z14JX

Purified rabbit skeletal muscle myosin in 0.6M KCl was adsorbed onto mica flakes, quick frozen by contact with a liquid helium-cooled copper block in a Heuser-type cryopress (Heuser JE, J. Mol.Biol. 1983. 169:155-195). The sample was freeze-fractured and deep-etched in a Balzers 400 freeze fracture device, and rotary-replicated in Pt-C at 11 degrees.

This image was recorded at 150,000x in a JEOL 100CX2 transmission electron microscope operating at 100 kev, on Kodak 4489 film. The image was digitized using a Nikon Coolscan 9000ED scanner at 20 micron pixel size.

• 2021

• Ip, Wallace

Preparation: unfixed tissue; freeze_fracture/freeze_etch; whole mounted tissue
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data

• Cell Image Library Group ID: 1132

• Oryctolagus cuniculus

• Cytoskeleton
• Skeletal muscle cell

• ATPase activity, coupled to movement of substances
• Skeletal muscle contraction

• data
• image

• No linguistic content; Not applicable

Samplenumber: 4659

Doi: https://doi.org/10.6075/J02Z14JX

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL4659

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12