Christensen, Søren T.; Schneider, Linda; Cammer, Michael (2021). CIL:35213, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00G3J2X

Time lapse movie of mouse embryonic fibroblasts in culture imaged at 30 second intervals by phase contrast microscopy. A micropipette is positioned near the cells to deliver either a growth factor or control buffer. In this sequence a buffer is added cells move independently of the addition.

• 2021

• Cammer, Michael
• Christensen, Søren T.
• Schneider, Linda

These data were collected as research which led to the published article Cell Physiol Biochem 2010;25:279-292. Microscopy was performed with a 10X planapo phase contrast objective on an Olympus IX 70 microscope with a SensicamHQ cooled CCD camera and a heated stage insert set to 37 degrees C. Cells were grown and serum starved for 48 h in a coverslip bottom MatTek dish. Small amounts of PDGF-AA were continuously ejected from the micropipette to create a gradient in the vicinity of growth-arrested wt MEFs. Cell movement was monitored with time lapse video microscopy, taking images at 30 second intervals. A Femtojet Micromanipulator 5171 (Eppendorf-Brinkman Instruments) and a pump (model Femtojet; Eppendorf) were used to control the position of the micropipette and the pressure
required for the chemoattractant flow. Femptotip II micropipettes were positioned within 1 μm
of the cover slip and pressure set at 30 to 45 hPa. Spatial scale is approximate.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 8539

• Mus musculus

• Fibroblast
• Ruffle

• Cell motility
• MEF
• Receptor signaling protein tyrosine kinase activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35213

Doi: https://doi.org/10.6075/J00G3J2X

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35213

Reference

• Cell Physiol Biochem 2010;25:279-292 https://www.ncbi.nlm.nih.gov/pubmed/?term=20110689

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12