Allen, Richard (2021). CIL:34601, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WH2NZV

High resolution micrograph of a cross section the 9 + 2 axoneme of the cilium of Tetrahymena. The plasma membrane covers the axoneme and is continuous with the plasma membrane covering the cell body. Each doublet tilts inward to the tangent of the axonemal cylinder. Two arms, an inner arm and outer arm, extend from the complete microtubule of each doublet. These arms have ATPase activity and are called dyneins. They cause one doublet to slide against the adjacent doublet. Other protein links in the axoneme are elastic and induce the characteristic bending of the cilium and prevent excess sliding as the doublets slide past each other. TEM taken on 7/15/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 9702

• Tetrahymena pyriformis

• Axonemal dynein complex
• Axonemal microtubule
• Axoneme
• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist

• Axonemal organization
• Cilium axoneme assembly

• data
• image

• No linguistic content; Not applicable

Samplenumber: 34601

Doi: https://doi.org/10.6075/J0WH2NZV

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34601

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2022-08-12