Porter, Keith R.; Claude, Albert; Fullam, Ernest F. (2021). CIL:37242, Gallus gallus, fibroblast, CEF (chicken embryo fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QV3K6F

Classic TEM. First description of the endoplasmic reticulum. Whole mount of unfixed, dried chick embryo fibroblast. Embryonic chick cells were grown on a thin collodion film supported by a fine mesh copper grid. After the cells had grown and spread out thin processes, the preparation was air dried. They reasoned that the thin processes would allow electrons to penetrate in the electron microscope. The only microscope available to them was owned by the Interchemical Corp. and housed in the Empire State Building. Fullam was the technician. The electron microscope was a first generation RCA type B. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

• 1952-12-12

• 2021

• Claude, Albert
• Fullam, Ernest F.
• Porter, Keith R.

Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi.

Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain

• Cell Image Library Group ID: 9996

• Gallus gallus

• CEF (chicken embryo fibroblast)
• Endoplasmic reticulum
• Fibroblast

• data
• image

• No linguistic content; Not applicable

Samplenumber: 37242

Doi: https://doi.org/10.6075/J0QV3K6F

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL37242

Reference

• J. Exp. Med. 81:233-246. 1945 https://www.ncbi.nlm.nih.gov/pubmed/?term=19871454

Other resource

• George E. Palade EM Slide Collection: http://cushing.med.yale.edu/gsdl/cgi-bin/library?c=palade&a=d&d=DpaladePanMorphI

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2023-08-17