Maddox, Paul S.; Hyndman, Francie; Monen, Joost; Oegema, Karen; Desai, Arshad (2021). CIL:28779, Caenorhabditis elegans, embryo. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MS3RDK

Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 in which CeCENP-A has been depleted by RNAi in strain OD31. Diffuse nuclear GFP signal is evident during prophase. However, unlike the control (CIL 28778), no discrete GFP foci were seen until anaphase onset, at which point foci of GFP-KNL-2 formed along the spindle and on the chromatin. The video is ∼18 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals.

• 2021

• Desai, Arshad
• Hyndman, Francie
• Maddox, Paul S.
• Monen, Joost
• Oegema, Karen

Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP–γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD camera (Orca ER; Hamamatsu) binning 2 × 2. Strain OD31 expressing GFP–KNL-2 was imaged in the same manner without the 1.5× auxiliary magnification.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11164

• Caenorhabditis elegans

• Chromatin
• Embryo
• KNL-2
• Spindle

• Anaphase
• CeCENP-A depletion
• Mitosis
• Prophase

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 28779

Doi: https://doi.org/10.6075/J0MS3RDK

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL28779

Reference

• J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5. https://www.ncbi.nlm.nih.gov/pubmed/?term=17339379

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2022-08-12