Svitkina, Tatyana M.; Verkhovsky, Alexander B.; Borisy, Gary G. (2021). CIL:23051, Homo sapiens, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05D8QKG

MAP4 is not required for plectin binding to microtubules (MTs). Electron microscopy of control human 356 fibroblasts (CIL:23051, this image) and MAP4 immunodepleted (CIL:23052) cells after staining with antibody to NH 2 terminus of MAP4. Cells were microinjected with an antibody to the COOH-terminal domain of MAP4 to remove MAP4 from MTs. 10-nm gold particles decorate MTs in control cells, but are absent in injected cells. Plectin forms bridges between intermediate filaments and MTs in both cells. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 9c in J Cell Biol. 1996 Nov;135(4):991-1007.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.
• Verkhovsky, Alexander B.

Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; tannic acid
Relation to intact cell: platinum replica
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: uranyl salt; shadowing and plating; primary antibody plus labeled secondary antibody; immunogold
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8924

• Homo sapiens

• Fibroblast
• Intermediate filament
• Microtubule
• Plectin

• Human 356 fibroblasts

• data
• image

• No linguistic content; Not applicable

Samplenumber: 23051

Doi: https://doi.org/10.6075/J05D8QKG

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL23051

Reference

• J Cell Biol. 1996 Nov;135(4):991-1007 https://www.ncbi.nlm.nih.gov/pubmed/?term=8922382

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2022-08-12