CIL:40197, Mus musculus, CNS neuron (sensu Vertebrata), astrocyte of the cerebellum
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- Collection
- Cite This Work
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Cone, Angela; Sosinsky, Gina; Martone, Maryann (2021). CIL:40197, Mus musculus, CNS neuron (sensu Vertebrata), astrocyte of the cerebellum. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0736R2X
- Description
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Mosaic image of mouse cerebellum reconstructed through automated acquisition and stitching of over 1700 individual tiles in the X, Y and Z planes, triple labeled for astrocytes (GFAP; Red), gap junctions (Connexin 43; Green) and nuclei (DAPI; Blue), and imaged using multiphoton microscopy. The contrast and brightness were adjusted on the thumbnail image for display purposes. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
- Date Issued
- 2021
- Researchers
- Methods
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Mouse brain was perfused with 4% paraformaldehyde, sectioned through the cerebellum in the sagittal plane on a vibratome (50 µm). Tissue sections were immunostained using Rabbit Cx43 antibody (Sigma) 1:600, and Chicken GFAP antibody (Neuromics) 1:500, 72hour incubation at 4C. Secondary antibodies were Donkey anti rabbit-Al488, and Donkey anti-chicken-RRX, 2.5hour incubation at room temperature, followed by staining with DAPI. Sections were mounted in gelvatol. Individual images were gathered using a Nikon PlanApo 60X NA 1.45 objective on a BioRad RTS2000 multiphoton microscope. The mosaic image was generated by tiling 37 (X) x 48 (Y) maximum projection images.
- Technical Details
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Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: multi-photon microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488; X-Rhodamine; 4',6-diamidino-2-phenylindole (DAPI)
Data qualification: Processed - Series
- Scientific Name
- Anatomy
Formats
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40197
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40197
- Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J01C1WQ3
Source data
Previous version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12